Istrazivanja i projektovanja za privreduJournal of Applied Engineering Science


DOI: 10.5937/jaes18-25651
This is an open access article distributed under the CC BY-NC-ND 4.0 terms and conditions. 
Creative Commons License

Volume 18 article 686 pages: 262 - 266

Muhammad Alfian Mizar*
Universitas Negeri Malang, Faculty of Engineering, Malang, Indonesia

Mohamad Amin
Universitas Negeri Malang, Faculty of Mathematics and Natural Sciences, Malang, Indonesia

Mokhamad Sholihul Hadi
Universitas Negeri Malang, Faculty of Engineering, Malang, Indonesia

Muhammad Aziz
The University of Tokyo‚ Institute of Industrial Science, Tokyo, Japan

Hasanuddin University, Faculty of Mathematics and Natural Sciences, South Sulawesi, Indonesia

Preservation of fossil fuels are currently depleting with the massive exploitation of fuels. In this condition, breakthroughs are necessary to produce alternative fuels. One of the breakthroughs is bioethanol. It is a renewable energy which is more effective than gasoline inasmuch it can increase combustion efficiency and reduce exhaust emissions. In this work, a bioethanol process was done by using sugarcane bagasse waste material which has a lot of lignin and cellulose content. The content was converted into bioethanol by utilizing a strong base to degrade lignin and T. Viride as cellulose-producing and S. cereviseae yeast as a sugar converter to bioethanol. This present research aims to find the best formula of bioethanol production based on sugarcane bagasse with variations in cellulose hydrolysis temperature, shaking speed, and fermentation time by using an integrated shaker machine fuzzy-logic control of temperature and humidity. This research employed a complete randomized design experimental research (CRD) to test temperature, speed, and time modification by using a shaker machine. The independent variables were: (1) temperature, (2) shaking speed, and (3) fermentation time. The dependent variables measured were reducing sugar and bioethanol levels. The results showed that the best formula for producing bioethanol levels was at a treatment temperature of 45 °C and a speed of 140 rpm with fermentation time of 48 hours which resulted in a bioethanol level of 2.75%.

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The authors would like to thank greatfully to Universitas Negeri Malang for supporting the research funding. We are also thank you to head and the researcher and member molecular biology working group for the technical support.

1. Sulfahri, Ni’matuzahroh, and Manuhara S. W. (2016) Studies on The Hungate technique for ethanol fermentation of algae Spirogyra hyalina using Saccharomyces cerevisiae, Biofuels

2. Sulfahri, Amin M., Sumitro S. B., and Saptasari M., (2016) Bioethanol production from algae Spirogyra hyalina using Zymomonasmobilis,Biofuels, 7(6).

3. Chen W. et al. (2017) Journal of the Taiwan Institute of Chemical Engineers Producing bioethanol from pretreated-wood dust by simultaneous saccharification and co-fermentation process. 79:43-48

4. Pradana Y. S., Hartono M., Prasakti L., and Budiman A. (2019) Effect of calcium and magnesium catalyst on pyrolysis kinetic of Indonesian sugarcane bagasse for biofuel production. Energy Procedia, 158:431–439.

5. de Araujo Guilherme A., Dantas P. V. F., de AC. E. Padilha, dos Santos E. S., and de Macedo G. R. (2019) Ethanol production from sugarcane bagasse: Use of different fermentation strategies to enhance an environmental-friendly process, J. Environ. Manage, 234:44–51.

6. Chen W., YeS., and Sheen H. (2012) Hydrolysis characteristics of sugarcane bagasse pretreated by dilute acid solution in a microwave irradiation environment. Appl. Energy, 93:237–244.

7. Delabona S., Sanchez C., Ribeiro M., Freitas S., and Geraldo J. (2012) Bioresource Technology Use of a new Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production. Bioresour. Technol, 107:517–521.

8. Lee S., Kim M., Kim S., Hong C., Ryu S., and Choi I. (2016) Transcriptomic analysis of the white rot fungus Polyporusbrumalis provides insight into sesquiterpene biosynthesis, 182:141–149.

9. Carolina A. et al. (2019) Biocatalysis and Agricultural Biotechnology Catalytic properties of xylanases produced by Trichoderma piluliferum and Trichoderma viride and their application as additives in bovine feeding. 19.

10. Copete-pertuzL. S., Alandete-novoa F., Placido J., Correa-londono G. A., and Mora-martinez A. L., (2019) Science of the Total Environment Enhancement of ligninolytic enzymes production and decolourising activity in Leptosphaerulinasp . by co – cultivation with Trichoderma viride and Aspergillus terreus. Sci. Total Environ., 646:1536–1545.

11. Haghighi S., Hossein A., and Tabatabaei M., (2013) Lignocellulosic biomass to bioethanol , a comprehensive review with a focus on pretreatment, 27:77–93.

12. Pongcharoen P., Chawneua J., and Tawong W., (2018) High temperature alcoholic fermentation by new thermotolerant yeast strains Pichia kudriavzevii isolated from sugarcane fi eld soil. Agric. Nat. Resour., 52(6):511–518.

13. Tian S., Zhao R., and Chen Z. (2017) Review of the pretreatment and bioconversion of lignocellulosic biomass from wheat straw materials. Renew. Sustain. Energy Rev., 91:pp. 483–489.

14. Waheed A. et al. (2017) Insight into progress in pre-treatment of lignocellulosic biomass. Energy, 122: 724–745.

15. Chuck‚ C. J., Parker H. J., Jenkins R. W., and Donnelly J. (2013) Bioresource Technology Renewable biofuel additives from the ozonolysis of lignin.Bioresour. Technol., 143:549–554.

16. Jahnavi G., Prashanthi G. S., Sravanthi K., and Rao L. V. Status of availability of lignocellulosic feed stocks in India : Biotechnological strategies involved in the production of Bioethanol,” Renew. Sustain. Energy Rev., 73:798–820.

17. Zhuang X. et al. (2016) Bioresource Technology Liquid hot water pretreatment of lignocellulosic biomass for bioethanol production accompanying with high valuable products. Bioresour. Technol., 199:68–75.

18. Byadgi S. A. and Kalburgi P. B. (2016) Production of Bioethanol from Waste Newspaper. Procedia Environ. Sci., 35:555–562.

19. Luong C., Duy L., and KameiI. (2018) International Biodeterioration & Biodegradation The improvement of sodium hydroxide pretreatment in bioethanol production from Japanese bamboo Phyllostachys edulis using the white rot fungus Phlebiasp . MG-60.Int. Biodeterior. Biodegrad., 133:86–92.